VTNE Laboratory Procedures Practice Test Questions Answers

Last Updated on March 8, 2025

VTNE Laboratory Procedures Practice Test Questions Answers 2025. Another group of questions on the VTNE involves laboratory procedures. These questions comprise 15 percent (30 items) of the questions on the exam. These questions test your knowledge of collecting specimens or samples, preparing samples for lab tests, performing the lab tests and procedures, and maintaining safety in the lab.

Laboratory procedure questions may ask you to identify the correct definition of a named procedure or select the proper use of a particular tool or equipment. You might have to determine a correct or incorrect statement among four choices. You may also encounter questions that describe a scenario and ask you how the technician in the description should proceed.

Some laboratory procedure questions focus on the microscope, one of the most commonly used equipment in veterinary laboratories. You may be asked about specific parts of a microscope (e.g., where a part is located or its function) or how to care for a microscope (e.g., how it is stored and cleaned).

VTNE Laboratory Procedures Practice Test

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VTNE Laboratory Procedures Practice Test

VTNE Laboratory Procedures Practice Test
Total Questions - 25
Time Limit: N/A

To answer these types of questions, you have to rely on what you know about anatomy, bodily fluids, and common laboratory tests and procedures. You may be asked questions about the process of gathering specimens (e.g., drawing blood, collecting urine or feces, retrieving parasites, etc.) or you may be asked about the results of laboratory tests (e.g., what the sample would look like if it tested positive for the presence of a specific disease).

1 / 25

1) When using a centrifuge, which practice is essential for safety and sample integrity?

A) Running only one tube per cycle

B) Overfilling the tubes to ensure maximum yield

C) Balancing the tubes with an equal volume of sample in opposite buckets

D) Operating at maximum speed regardless of sample type

2 / 25

2) When performing a fecal flotation test, it is important to:

A) Use a high–speed centrifuge to concentrate parasite eggs

B) Immediately examine the slide without any mixing

C) Mix the fecal sample with a solution of appropriate specific gravity

D) Use distilled water as the flotation solution

3 / 25

3) Proper cleaning and maintenance of a compound microscope in the laboratory should include:

A) Wiping the lenses with a paper towel

B) Avoiding cleaning the eyepiece to preserve light intensity

C) Removing the objective lenses for cleaning with soap and water

D) Using a lens cleaning solution and a microfiber cloth for the optics

4 / 25

4) Quality control samples in a laboratory are used primarily to:

A) Monitor and ensure the accuracy of test results

B) Replace patient samples when insufficient volume is obtained

C) Train new technicians

D) Expedite test processing during emergencies

5 / 25

5) During a chemical spill in the laboratory, the technician should first:

A) Notify the supervisor and evacuate the area if necessary

B) Continue working if the spill appears minor

C) Immediately clean the spill without alerting others

D) Cover the spill with a cloth to prevent further spread

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6) When storing biological samples for future testing, it is critical to:

A) Store samples at room temperature to prevent freezing

B) Label samples with only the animal’s species

C) Clearly label each sample with the date, time, and identification details

D) Mix different samples together to conserve storage space

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7) All the following are common anticoagulants except:

A) zeolite

B) EDTA

C) sodium citrate

D) heparin

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8) Which of the following technicians is violating laboratory safety procedures?

A) Joseph wears a protective coat, disposable gloves, and goggles while working with chemical agents.

B) Amanda accidentally ingests a biological agent and seeks the aid of the senior lab technician.

C) Tiffany breaks a beaker of toxic substances, cleans up the spill and broken glass, then returns to work.

D) Bill uses small- and medium–sized containers instead of large containers to store chemicals.

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9) A postprandial urine sample would be taken after a period of:

A) activity

B) fasting

C) rest

D) eating

10 / 25

10) A gray and red Vacutainer tube contains:

A) thrombin

B) heparin

C) silicone serum separation material

D) silicone coating with no additive

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11) A healthy feline should have a red blood cell count ranging from:

A) 5.5–9.5 × 10^6/mm³

B) 7.0–13.0 × 10^6/mm³

C) 5.5–10.0 × 10^6/mm³

D) 5.0–8.5 × 10^6/mm³

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12) When performing venipuncture on a cat using the left femoral vein, the patient should be placed in:

A) dorsal recumbency

B) sternal recumbency

C) left lateral recumbency

D) right lateral recumbency

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13) The purpose of a Bunsen burner in a laboratory is primarily to:

A) Warm up laboratory reagents gradually

B) Provide a controlled flame for heat–treating samples or melting reagents

C) Generate smoke for chemical reactions

D) Sterilize instruments through dry heat

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14) Which of the following is a common pre–analytical error in laboratory testing?

A) Incorrect storage temperature of specimens

B) Use of calibrated equipment

C) Following standard operating procedures

D) Proper labeling of samples

15 / 25

15) Arthrocentesis is the method used to collect:

A) abdominal fluid

B) synovial fluid

C) thoracic fluid

D) cerebrospinal fluid

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16) A veterinarian collects a blood sample from a ferret to check the animal’s WBC count. This sample will provide information about the ferret’s:

A) white blood cells

B) whole blood culture

C) white blood culture

D) whole blood cells

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17) The proper procedure for disposing of sharps in the laboratory is to:

A) Dispose of needles in a designated sharps container without recapping

B) Recap needles immediately after use and place them in the regular trash

C) Wash and store the needles for potential reuse

D) Bend the needles before disposal

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18) The majority of clinical waste disposal containers are which color?

A) Purple

B) Green

C) Blue

D) Red

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19) When preparing a blood smear for microscopic examination, the correct technique involves:

A) Blotting the blood with filter paper to remove excess sample

B) Using a frosted slide to label the specimen and then air-drying the smear

C) Immediately fixing the smear with methanol before stainingImmediately fixing the smear with methanol before staining

D) Spreading a drop of blood directly on the slide without a cover slip

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20) Hemolysis is a form of cell disintegration in:

A) white blood cells

B) platelets

C) red blood cells

D) plasma

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21) During a laboratory accident involving exposure to a toxic chemical, the technician should:

A) Wait to see if symptoms develop before taking any action

B) Neutralize the chemical with a corresponding neutralizer without rinsing

C) Immediately rinse the affected area with water for at least 15 minutes

D) Continue working and report the incident at the end of the day

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22) When preparing reagents for a chemistry panel, it is essential to:

A) Follow the manufacturer’s guidelines and use proper dilution techniques

B) Store all reagents at room temperature regardless of instructions

C) Combine reagents from different kits to reduce costs

D) Mix reagents in an unclean container to expedite the process

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23) When using pipettes in the laboratory, which practice helps prevent cross–contamination?

A) Blowing out the contents to clean the tip for reuse

B) Wiping the outside of the pipette with a cloth between samples

C) Using disposable pipette tips for each sample

D) Reusing the same pipette tip after rinsing with water

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24) In the context of urinalysis, the proper procedure for collecting a mid–stream urine sample from a canine patient is to:

A) Collect the sample after the urine has pooled on the floor

B) Collect the sample immediately at the start of urination

C) Use a free–catch method after the patient has begun urinating

D) Catheterize the patient regardless of gender

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25) For cytology slide preparation, which of the following is the best practice?

A) Smearing a thick layer of sample on the slide

B) Covering the smear with a heavy liquid fixative immediately

C) Ensuring a thin and even smear to allow proper cell examination

D) Air–drying the smear for 24 hours before staining

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